There are different ways to obtain ADSCs, i.e., enzymatic and non-enzymatic.In enzymatic method, the collagenase is added to lipoaspirate and is then incubated. Once the fat is dissolved, the tube is centrifuges and a ‘pallet’ of concentrated stem cells are found in the bottom. The amount is low in quantity but highest in concentration.
In non-enzymatic method, the lipoaspirate is subjected to centrifugation. The lowest and uppermost parts are discarded. The middle part contains endothelial cells, fibroblasts, lymphocytes, monocytes, macrophages, pericytes and ADSCs. There are certain terms which are associated with the concept of ‘stem cells’. The term SVF (Stromal Vascular Fraction) is normally used with stem cells. The SVF is a heterogenous cell mixture which include tissue macrophages, smooth muscle cells, T and B-lymphocytes, endothelial cells, pericytes, myocytes, fibroblasts and ADSCs. It is normally prepared by enzymatic digestion of lipoaspirate. SVF is at the lowest part after centrifugation.
Swelling and bruising usually subsides within 1 week. The final results are visible after 3 – 6 months.